In-vivo Conditions for Live Cell Assays

Many physiological processes take place under flow conditions: Blood flowing through the vasculature; cancer cells circulating throughout the body; plaque forming on teeth under the presence of saliva flow. It is now well established that physiological flow has a profound impact on many biological studies, yet a lot of research is still studied in vitro without the presence of flow. The BioFlux System gives you the ability to introduce flow to your research and drug discovery experiments effectively emulating in vivo conditions and getting closer to understanding what’s happening under more physiological conditions. 

Offering the physiological relevance of laminar flow chambers with the throughput and convenience of standard well plates, BioFlux systems have many features and benefits including:

  • High Speed: With automated experimental control, multiplexing up to 96 simultaneous experiments, and sophisticated data analysis software simplifying and accelerating complex functional assays, large projects can be effectively reduced from months to days. 
  • High Quality: Glass bottom plates with superior image quality from microscopes and high content imagers provide unparalleled image quality. 
  • Simple to Use: Well plate microfluidic design improve reliability and reproducibility while enabling precise control of critical parameters.
  • Modular Design: A BioFlux system can be added to any existing inverted microscope setup instantly expanding its capabilities.

BioFlux systems come in different configurations as described below and have been featured in multitudes of publications, presentations and webinars.

BioFlux enables a wide variety of cell-based assays in the fields of immunology, vascular biology, microbiology, cancer research, stem cells, and more. Many of these applications benefit from the ability to create a physiologically-relevant shear flow profile, such as platelet adhesion, cell adhesion, rolling velocity, transmigration and stem cell differentiation. Other applications can benefit from the ability to introduce compounds in a time-resolved and well-controlled manner, such as wound healing, pharmacology and neurite-outgrowth assays.

Modular Systems

BioFlux 200: The Primary System

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The BioFlux 200 System is a benchtop instrument which enables up to 24 temperature-controlled flow cell assays in parallel. The BioFlux Pressure Interface connects a highly precise and accurate electropneumatic pump to the well plates to initiate controlled shear flow. The system works with your existing inverted microscope and is compatible with fluorescence, bright field, phase, and confocal imaging. User-friendly software automates experimental controls and provides a powerful analysis package.

The Pressure Interface mounts on top of the BioFlux Plates and sits on an inverted microscope.

The BioFlux Controller connects to the Pressure Interface and controls shear flow, temperature and flow direction.

BioFlux Plates are SBS-standard well plates with integrated flow cells that can be loaded using pipettes or liquid handling workstations. The bottom of each flow cell is formed with a 180μm cover slip for optimal imaging.

BioFlux Software offers complete control over experimental conditions, including dynamic control over shear flow changes

BioFlux 1000z  →  The Fully Integrated System


The BioFlux 1000z systems offer a fully-integrated solution for automated, high throughput shear flow assays. High performance microscopy components are seamlessly integrated with BioFlux controls to offer the ultimate in speed, precision and convenience. BioFlux 1000z ships as a turn-key imaging workstation for live cell assays under flow. The system includes the following primary components, but can be customized to meet your application needs:

  1. BioFlux Controller and Well Plate Interface
  2. High resolution inverted microscope
  3. Automated stage
  4. Automated fluorescence module
  5. Autofocus module
  6. High resolution CCD Camera
  7. PC Workstation
  8. Additional options: environmental control, vibration isolation table

BioFlux EZ  →  The Affordable System

All the benefits of the industry leading BioFlux cell analysis platform, now in a highly affordable configuration. Need to run flow cell experiments, but don't need 2-phase flow or high throughput? TheBioFlux EZ is the solution; it provides all the basic benefits of the industry standard BioFlux system, at a price point similar to a "home brew" flow cell setup.

The BioFlux EZ  can run up to 6 simultaneous flow cell experiments and is ideal for platelet aggregation, leukocyte adhesion, and biofilm analysis. As with all BioFlux systems, the EZ works with an inverted microscope and is compatible with fluorescence, brightfield, phase and confocal imaging. User-friendly software automates experimental protocols and provides a powerful analysis package. Upgrade capabilities allowing for 2-phase flow and/or higher throughput make the BioFlux EZ the perfect choice for your functional assay research.


New! BioFlux Touch  →   The Touch Enabled System


BioFlux Touch makes it possible to program and control your microfluidic assays by means of a convenient touch interface, freeing up valuable bench space and minimizing contamination of your work environment.
The compact nature of the system allows it to fit inside a hood or take up minimal bench space. The intuitive touch enabled software provides a simple way to control multiple experiments at once. 

BioFlux Quattro  →   The High Throughput System


Achieve even higher speed in drug development under shear stress. With the BioFlux Quattro HT Array system, you can accelerate lead optimization while preserving relevant physiological conditions. The BioFlux Quattro comprises a 4-plate array that connects to one BioFlux controller. With the Quattro you can obtain quadruple your efficiency and decrease your experiment time while still keeping the ability to image plates on virtually any high content system (HCS) or automated microscope.

BioFlux Quattro is well suited for high throughput assays in cardiovascular, cancer, anti-microbial and immunology research and drug development.

BioFlux BeQuanti   →   The Cell Rolling Analysis System

BeQuanti (Behavior Quantification) is a software package designed for the automated detection and characterization of cells interacting under flow conditions. BeQuanti performs accurate analysis of several cell adhesion parameters, including:

  • Quantification of tethering and calculation of speed of rolling
  • Detection of the new sub-rolling metric
  • Classification of cellular events depending on duration of arrest
  • Discrimination between round, polarized and spread cells
  • Characterization of post-adhesion stabilization
  • Detection and analysis parameters are fully user customizable allowing for maximal analysis flexibility. Data analysis can be performed in real time and can be provided in several different output formats, including frame-by-frame data as well as statistical data. 

Due to its modular architecture, the package can be easily expanded to allow cell detection and analysis under different experimental conditions. 

BeQuanti is not only a great addition to any BioFlux System, it is a stand-alone package that can analyze data from any cell adhesion platform.

The software is available for Mac OS X (intel) and for Microsoft Windows 7.x


Download a 30 days trial of the software:

Before using the software for the first time, we recommend you view the quick tutorial below. Also we recommend you view the BeQuanti Webinar.

BeQuanti Tutorial

BeQuanti Webinar

Product Data Sheet


Starting window - movie import and analysis

Results window - cell behavior quantification

Results window - cell morphology analysis

Results window - table of cell behavior quantification


Product Support

Our products are backed by an excellent support system that includes extensive online support documents as well as the ability to ask technical questions directly to our technical team. Our Library and Support Center contains extensive product information as well as forms for submitting customer related questions. Wether you are a full BioFlux customer or simply interested in our product line, we recommend you give the support center a visit.