ONE FLEXIBLE SYSTEM - MANY ASSAYS
The IonFlux system, with its advanced fluidic control, plate-integrated recording and compound delivery, is capable of satisfying the needs of the most demanding ion channel screening assays. The speed of recording, managed by the parallel execution of all experimental patterns, married with the system's unique continuous flow system, provides the perfect platform for the study of ligand-gated as well as voltage-gated channels. Below are example of assays performed on the IonFlux System
The microfluidic well plate technology employed by the IonFlux system enables unlimited flexibility and synchronous compound additions across the full plate. This flexibility leads to increased throughput in difficult-to-execute ligand gated assays. Fast compound displacement and continuous perfusion dramatically improve ligand movement and washout, enabling the study of challenging targets such the allosteric modulators (open and closed pore), nACh receptors and NMDA receptors. Below are a few examples:
- Ion channel pharmacology under flow: automation via well-plate microfluidics.
- Recording of GABAA receptor-mediated Cl- currents
- Recording of nAChR alpha1 receptor currents
- Transiently Transfected Cell Lines for GABA Receptor Screening
- A GABA Ligand Gated Ion Channel Screen - Results and Best Practices
- Development and optimization of an automated electrophysiological assay for the neuronal a4b2 nicotinic receptor using the IonFlux 16
Voltage-gated ion channels are responsible for determining the shape, duration, and frequency of action potentials in excitable cells. Given this important physiological role they have been heavily-pursued targets in drug discovery efforts. Voltage-gated channels also control important physiological functions in non-excitable cells, for example secretory epithelial cells. The flexibility in fluidics and the parallel execution of experiments, decreases overall assay time to completion for voltage-gated assays. Continuous flow provides complete washouts and solution exchange, increasing the accuracy of recorded data. Below are a few examples.
hERG (KV 11.1)
Nav 1.7 and 1.8
- hERG K+ channel currents and pharmacology using the IonFlux system
- Temperature Dependent hERG Channel Pharmacology and Kinetics on the IonFlux System
- B’SYS CHO hERG+ Characterizeation and Functional Validation with IonFlux Single Mode HT.
- Nav 1.7 and 1.8 Recordings on the IonFlux System
- A-803467 Pharmacology Study on hNav1.8 and hNav1.7 Using the IonFlux System
- G-Seal, single cell vs. Ensemble recording: Validation of Nav assay formats
- Optimization of hERG screening using IonFlux HT automated system
- Development of automated patch clamp methods for Kir4.1 using the IonFlux HT