Single-cell analysis for CTCs

The IsoFlux System was introduced to provide high efficiency recovery of CTCs from a routine blood draw. Using an advanced microfluidic separation technology, the platform achieves tumor cell purities on the order of 1-5% using the IsoFlux CTC Enrichment Kit, and purities greater than 10% using the IsoFlux NGS DNA Kit. While these samples are suitable for most molecular assays, isolating single tumor cells from these pre-enriched samples offers two compelling advantages: (1) the ability to analyze circulating tumor material at 100% purity, and (2) the ability to characterize each cell individually, obtaining a profile of the heterogeneity present in the tumor cell population.

This application note presents validation results for two single-cell isolation methods, both using the IsoFlux as a necessary starting point for CTC enrichment.

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