IsoFlux - Liquid Biopsy System - Online Webinars
Attend live webinars about different research techniques being implemented with the IsoFlux Liquid Biopsy system.
All online Webinars are recorded for later viewing. Below is the latest presented.
Check this page regularly for upcoming online webinars, when one is scheduled, information and registration will appear below.
RECORDED ONLINE WEBINARS
SOMATIC MUTATION DETECTION FROM LIQUID BIOPSIES: THE ISOFLUX NGS WORKFLOW
Presented on November 3 2015
Speaker: Dr. Cristian Ionescu-Zanetti, CTO & Co-Founder Fluxion Biosciences
Introduction: Next generation sequencing (NGS) of blood-derived nucleic acids is an emerging paradigm for determining the mutational status of cancer patients over time. Here we present data from a CTC enrichment modality that results in tumor cell purities of >10% and a high sensitivity NGS data analysis workflow that enables the use of standard amplicon panels typically used for primary tissue.
Methods: Blood samples were enriched for CTCs using the IsoFluxTM System, from a starting blood volume of 7.5-14ml. Matched samples were enumerated to determine the CTC load, where CTCs were defined as CK+, CD45- nucleated cells (DAPI+). Cells were lysed and DNA was amplified using the IsoFlux NGS Kit (Fluxion Biosciences). Targeted libraries were sequenced using both the MiSeq (Illumina) and PGM (ThermoFisher) sequencing instruments; data was analyzed using a customized variant calling/filtering pipeline.
Results: Multisite analytical validation data, based on spiking of cells into whole blood, demonstrated a detection limit down to 2 cells/ml blood. Clinical data from two different clinical pilot studies demonstrates the detection of somatic variants for a majority of samples, and significant overlap between detected mutations and known somatic mutation sites. This assay makes possible the detection of somatic variants from urological cancer patients without the need for a tissue biopsy.
ISOFLUX WEBINAR - ISOFLUX NGS WORKFLOW FOR CTC
Presented December 2 2014
The IsoFlux System is the first instrument of its kind to capture rare circulating tumor cells (CTCs) with sufficient recovery and purity to meet the demands of Next Generation Sequencing (NGS) applications.
In this webinar, you'll see examples of how a complete workflow can be established in your own lab to track mutations in cancer samples, all using your own existing NGS pipeline.
Topics will include:
The promise of the blood biopsies in cancer management | Sample challenges for NGS applications | NGS workflow overview | Use of the AmpliSeq Cancer Hotspot Panel v2 | Optimization of the variant calling and filtering in Ion Reporter | Analytical validation of CTC NGS assay | Clinical patient data using NGS assay
Cristian Ionescu-Zanetti, CTO & Co-Founder Fluxion Biosciences
Christopher Davies, Director of Bioinformatics, IonTorrent / Thermo Fisher